More accurate analysis of maternal effect genes by siRNA electroporation into mouse oocytes
Takuto Yamamoto, Shinnosuke Honda, Issei Ideguchi, Motoki Suematsu, Shuntaro Ikeda, Naojiro Minami
This study introduces an electroporation method to suppress maternal effect genes in mouse oocytes without removing cumulus cells, showing efficient maternal mRNA suppression and revealing H3.3's role in post-fertilization development.
Journal of Reproduction and Development · Jan 1, 2023Peer-reviewedDevelopmental Biology