Shuntaro Ikeda

Long-read sequencing reveals transposable element-derived chimeric transcripts at zygotic genome activation in mammalian embryos

This study uses long-read sequencing to identify chimeric transcripts from transposable elements in mouse and bovine embryos during zygotic genome activation, revealing species-specific patterns.

Osteochondrogenic Transdifferentiation of Vascular Smooth Muscle Cells and Microenvironmental Dynamics in Medial Arterial Calcification

This paper establishes an OTAC mouse model to study medial arterial calcification, identifying osteochondrogenic transdifferentiation of vascular smooth muscle cells and associated microenvironmental changes using scRNA-seq.

Transgenic Bax gene efficiently induces lethality in mouse early embryos

This study compares the lethality-inducing potential of Bax, Casp3, and Casp9 in mouse early embryos and finds Bax most effective in causing embryo death.

Endogenous retrovirus-driven Pcgf5 plays critical roles in zygotic genome activation and noncanonical imprinting

This study identifies Pcgf5, a MERVL-regulated chimeric transcript, as critical for zygotic genome activation and noncanonical imprinting in mouse embryos.

Chimeric PRMT6 protein produced by an endogenous retrovirus promoter regulates cell fate decision in mouse preimplantation embryos

The paper identifies a chimeric PRMT6 protein produced by MERVL in mouse embryos that promotes cell proliferation and differentiation, differing from the canonical PRMT6 protein.

Analysis of histone H3K4me3 modifications in bovine placenta derived from different calf-production methods

The paper profiles genome-wide histone H3K4me3 modifications in bovine placentae from different calf-production methods and identifies differential modifications linked to developmental processes.

Diagnostic histone modification analysis of individual preimplantation embryos

The paper introduces WOW-CAT, a simplified version of NTU-CAT for histone modification analysis in preimplantation embryos, showing improved accuracy and applicability for epigenetic diagnosis.

MYC is essential for induction of major ZGA and subsequent preimplantation development

This study shows that Myc is essential for zygotic genome activation (ZGA) and preimplantation development in mouse embryos.

More accurate analysis of maternal effect genes by siRNA electroporation into mouse oocytes

This study introduces an electroporation method to suppress maternal effect genes in mouse oocytes without removing cumulus cells, showing efficient maternal mRNA suppression and revealing H3.3's role in post-fertilization development.

Genome-wide profiling of histone H3K4me3 and H3K27me3 modifications in individual blastocysts by CUT&Tag without a solid support (NON-TiE-UP CUT&Tag)

The paper introduces a modified CUT&Tag method for analyzing histone modifications in single blastocysts, showing agreement with ChIP-seq but with some limitations.

Comparative analysis of histone H3K4me3 modifications between early embryos and somatic tissues in cattle

The paper compares H3K4me3 histone modifications in bovine early embryos and adult somatic tissues to identify common and tissue-specific features.