Sensitive, high-throughput HLA-I and HLA-II immunopeptidomics using parallel accumulation-serial fragmentation mass spectrometry
K. Phulphagar, C. Ctortecka, S. Vaca, S. Klaeger, E. K. Verzani, G. M. Hernandez, N. Udeshi, K. R. Clauser, J. Abelin, S. A. Carr
This paper develops a high-throughput, sensitive MS-based immunopeptidomics workflow to identify HLA-I and HLA-II peptides from tumor samples, achieving improved coverage with reduced sample requirements.
bioRxiv · Mar 10, 2023Immunology