Genome editing in plants using the compact editor Cas{Phi}
Z. Li, Z. Zhong, Z. Wu, P. Pausch, B. Al-Shayeb, J. Amerasekerae, J. A. Doudna, S. E. Jacobsen
This paper demonstrates that the hypercompact Cas{Phi} nuclease can generate heritable gene edits in Arabidopsis with high specificity and efficiency, and shows that its performance is influenced by chromatin environment and temperature.
bioRxiv · Oct 31, 2022Plant Biology