A CRISPR/Cas12a-assisted platform for identification and quantification of single CpG methylation sites
J. E. van Dongen, J. T. W. Berendsen, J. C. T. Eijkel, L. I. Segerink
This paper introduces a CRISPR/Cas12a-based method to detect and quantify single CpG methylation sites using methylation-sensitive restriction enzymes, demonstrating its application in cancer gene analysis.
bioRxiv · Apr 6, 2021Biochemistry