Fast bioluminescent nucleic acid detection using one-pot isothermal amplification and dCas9-based split luciferase complementation
H. J. van der Veer, E. A. van Aalen, C. M. S. Michielsen, E. T. L. Hanckmann, J. Deckers, M. M. G. J. van Borren, J. Flipse, A. J. M. Loonen, J. P. H. Schoeber, M. Merkx
The paper develops a bioluminescent nucleic acid detection platform (LUNAS) integrated with RPA for rapid, sensitive, and portable diagnostics of SARS-CoV-2 RNA.
bioRxiv · Sep 12, 2022Bioengineering