High-throughput direct screening of restriction endonuclease using microfluidic fluorescence-activated drop sorter based on SOS response in E. coli
Y. Zhang, J. J. Agresti, Y. Zheng, D. A. Weitz
The paper develops a high-throughput method to screen restriction endonucleases using microfluidics and bacterial SOS response, achieving 30-fold enrichment of target genes in one hour.
bioRxiv · Jan 9, 2022 · PreprintBioengineering