Resolving subcellular pH with a quantitative fluorescent lifetime biosensor
J. J. Rennick, C. J. Nowell, C. W. Pouton, A. P. R. Johnston
This paper introduces pHLIM, a method to quantify sub-cellular pH using fluorescent lifetime imaging and deep learning, demonstrating its application in measuring pH changes in cellular compartments.
bioRxiv · Apr 13, 2022Cell Biology