First crystal structure of a non-canonical amino acid linked to a paramagnetic lanthanide tag facilitates protein structure determination using NMR-derived restraints
E. N. N. Okwei, S. Ganguly, H. L. Darling, J. M. Harp, A. Gulsevin, I. Coin, H. Mchaourab, K. Ledwitch, G. Kuenze, J. Meiler
The paper presents the first crystal structure of a non-canonical amino acid linked to a paramagnetic lanthanide tag, showing how it improves NMR-derived structural modeling of proteins.
bioRxiv · Apr 10, 2022 · PreprintBiophysics