Pooled single-cell CRISPRa/i screens for functional genomics in bacteria at scale
Jacob Brandner, Quoc Tran, Yujia Huang, Dmitry A. Sutormin, Karl D. Gaisser, Georg Seelig, Jesse G. Zalatan, James M. Carothers, Anna Kuchina
The paper introduces mapSPLiT, a pooled CRISPRa/i screening platform for bacteria, linking perturbations to single-cell transcriptomes and expanding regulatory networks in E. coli and P. putida.
bioRxiv · Dec 22, 2025 · PreprintGenomics