GenomicsbioRxivPreprint
Pooled single-cell CRISPRa/i screens for functional genomics in bacteria at scale
Jacob Brandner, Quoc Tran, Yujia Huang…·Anna Kuchina
The paper introduces mapSPLiT, a pooled CRISPRa/i screening platform for bacteria, linking perturbations to single-cell transcriptomes and expanding regulatory networks in E. coli and P. putida.