Female iPSC X-chromosome inactivation (XCI) erosion and its transcriptomic effects during CRISPR gene editing and neural differentiation
Christina Thapa, Emily Oh, David Sirkin, Jennifer Lahey, Sol Díaz de León‐Guerrero, Ada McCarroll, Prarthana Gowda, Hanwen Zhang, Alexandra Barishman, Lilia Peyton, Siwei Zhang, Rebecca M. Pollak, Ronald P. Hart, Carlos N. Pato, Anat Kreimer, , Jubao Duan
The paper investigates XCI erosion in CRISPR-edited female iPSCs and their derived neurons, showing variable erosion in iPSCs and preserved XIST effects on gene expression in neurons.
bioRxiv · Mar 1, 2026 · PreprintGenomics