Phylogenomics and metabolic engineering reveal a conserved gene cluster in Solanaceae plants for withanolide biosynthesis
Samuel Edward Hakim, Nancy Choudhary, Karan Malhotra, Peng Jian, Arne Bültemeier, Ahmed Arafa, Ronja Friedhoff, Maximilian Bauer, Jessica Eikenberg, Claus‐Peter Witte, Marco Herde, Philipp M. Heretsch, Boas Pucker, Jakob Franke
Comparing nine Solanaceae genomes reveals a conserved withanolide gene cluster; three of its P450s, expressed in yeast and N. benthamiana, oxidise 24-methyldesmosterol toward the withanoside V aglycone.
Nature Communications · Jul 10, 2025Peer-reviewedPlant Biology✦ In-depth digest