Robotic Handling Preserves Induced Pluripotent Stem Cell Derived Vascular Smooth Muscle Cells Differentiation using a Weekend-free, Automatable, Low-variability, Low-cost (WALL) Protocol
Monique Bax, Jeya Ramalingam, Valentin Romanov, Muhammad A Alsherbiny, Keerat Junday, Jordan Thorpe, Emily Hurley, Melanie Lim, Ingrid S. Tarr, Stephanie Hesselson, Eleni Giannoulatou, Renjing Liu, Adam Hill, Siiri E. Iismaa, Robert M Graham
This paper develops an automated protocol for differentiating iPSC-derived vascular smooth muscle cells and shows that automation does not compromise cell quality or protein expression.
bioRxiv · Nov 25, 2025 · PreprintDevelopmental Biology