High throughput detection of variation in single-cell whole transcriptome through streamlined scFAST-seq
Guoqin Sang, Jiaxin Chen, Meng Zhao, Huanhuan Shi, Jinhuan Han, Jiacheng Sun, Ying Guan, Xingyong Ma, Guangxin Zhang, Yuyan Gong, Yi Chen Zhao, Shaozhuo Jiao
This paper introduces scFAST-seq, a high-throughput single-cell full-length RNA sequencing method that improves detection of non-polyadenylated transcripts and RNA velocity, and enables simultaneous mutation and cellular status identification in tumor cells.
bioRxiv · Mar 19, 2023 · PreprintGenomics