The DNA event horizon in the Guaymas Basin subsurface biosphere: technical advances and re-defined limits in bulk extractions of nucleic acids from deep marine sediments
Gustavo A. Ramírez, Paraskevi Mara, David Beaudoin, Diana P. Bojanova, John E Hinkle, Brewster F. Kingham, Virginia P. Edgcomb, Yuki Morono, Andreas Teske
The paper evaluates DNA and RNA extraction protocols from Guaymas Basin sediments, showing that temperature and hydrothermal influence significantly impact nucleic acid recovery and microbial community analysis.
bioRxiv · Apr 15, 2024 · PreprintMicrobiology