Library preparation strategy critically impacts RNA virus sensitivity in clinical metagenomics
Dominika Stępniak, Bede Constantinides, Mia Weaver, Samantha Treagus, Samuel Wilkinson, Samuel Quarton, Mahboobeh Behruznia, Nicola Cumley, John Tyson, Alan McNally, Nicholas James Loman, Steven Pullan, Joshua Quick
This study shows that library preparation methods significantly affect RNA virus detection in clinical metagenomics, with SISPA-based approaches outperforming others in most scenarios.
medRxiv · May 21, 2026 · PreprintGenetic & Genomic Medicine