Two-timepoint assays of neural responses increase the sensitivity and specificity of single-cell whole-brain activity screens
Alejandro Ramirez, Evan J. Kyzar, Lydia Rogerson, Chloé Berland, Erica Rodriguez, Juan Guerrero, Ruby Setara, Maya Eisengart, Sophia Virkar, Luke Alexander Hammond, Anthony W. Ferrante, C. Daniel Salzman
This paper introduces a whole-brain screening method using two-timepoint assays to improve sensitivity and specificity in identifying neural responses, validated through studies on hunger, satiety, and semaglutide treatment.
Neuron · Jun 1, 2026Peer-reviewedNeuroscience