Recruitment of transcriptional effectors by Cas9 creates cis regulatory elements and demonstrates distance-dependent transcriptional regulation
Jubran Boulos, Arkadiy K. Golov, Nadav Keren, Itai Erlich, Nardeen Shehadeh, Nili Avidan, Hagai Kariti, Omer Sadeh, Lilac Haimovich-Caspi, Adam S. Helms, Noam Kaplan, Izhak Kehat
The paper shows that recruiting transcription factors to non-regulatory genomic sites can create enhancers or repressors, influencing gene expression at a distance with nonlinear dependence on site distance.
iScience · Nov 15, 2025Peer-reviewedGenomics