Proof-of-Concept of a DNA-Based Recording System for High-Throughput Functional Gene Screening
Shoichi Kato, Atsushi Ikemoto, Jun Isayama, Tetsuya Takimoto, Hideyuki Saya, Kenichi Hamada
The paper introduces PiER, a DNA-based system for functional gene screening that records perturbation-response events without single-cell isolation, and demonstrates its use in identifying WNT-related shRNA candidates.
ACS Synthetic Biology · Apr 15, 2026Peer-reviewedBioengineering