Label-free imaging of membrane potentials by intramembrane field modulation, assessed by Second Harmonic Generation Microscopy
Y. de Coene, S. Jooken, O. Deschaume, V. Van Steenbergen, P. Vanden Berghe, C. Van den Haute, V. Baekelandt, G. Callewaert, S. Van Cleuvenbergen, T. Verbiest, C. Bartic, K. Clays
This study demonstrates that second harmonic generation (SHG) from live cells is sensitive to membrane potential changes, offering a label-free method for measuring electrical activity.
bioRxiv · May 28, 2021Biophysics