Arrayed CRISPRi and Quantitative Imaging Describe the Morphotypic Landscape of Essential Mycobacterial Genes
T. J. de Wet, K. R. Winkler, M. M. Mhlanga, V. Mizrahi, D. F. Warner
The paper uses CRISPRi and imaging to analyze morphological changes in M. smegmatis, revealing gene function clusters and a new restriction-modification system.
bioRxiv · Mar 20, 2020Microbiology