Circular single-stranded DNA is a superior homology-directed repair donor template for efficient genome engineering
K. Xie, J. Starzyk, I. Majumdar, K. Rincones, T. Tran, D. Lee, S. Niemi, J. Famiglietti, B. Suter, R. Shan, H. Wu
This paper develops a non-viral system for efficient genome engineering using circular single-stranded DNA (cssDNA) donors, achieving high knock-in efficiency and improved safety compared to viral vectors.
bioRxiv · Dec 1, 2022 · PreprintBioengineering