Simple high-throughput encoding of deep mutational scanning libraries by oligo-based Golden Gate assembly
Kyrin R. Hanning, Emma J. Walker, Kevin Beijerling, Edward B. Irvine, James Jordan Steel, William Kelton
This paper introduces a method for high-throughput mutational library assembly using oligo-based Golden Gate assembly and validates it by improving nanobody affinity.
bioRxiv · Jul 16, 2025 · PreprintSynthetic Biology