Limitation of alignment-free tools in total RNA-seq quantification
Douglas C. Wu, Jun Yao, Kevin S. Ho, Alan M. Lambowitz, Claus O. Wilke
This study compares alignment-free and alignment-based RNA-seq pipelines for total RNA quantification, finding alignment-free tools less accurate for small and lowly-expressed RNAs.
BMC Genomics · Jul 3, 2018Peer-reviewedBioinformatics