Cryopreservation of cerebrospinal fluid cells preserves transcriptomics integrity for single-cell analysis
Mahesh Chandra Kodali, Jerry Antone, Eric B. Alsop, Rojashree Jayakumar, Khushi Parikh, Paula Sanchez-Molina, Bahareh Ajami, Steven Edward Arnold, Kendall Jensen, Sudeshna Das, Marc S. Weinberg
This study shows that cryopreservation of CSF cells using FBS/DMSO or Recovery media preserves transcriptomic integrity for single-cell analysis, enabling accurate cell type detection and gene expression profiling.
bioRxiv · Aug 10, 2023 · PreprintNeuroscience