This paper introduces SPEAR, a separation-free protein detection method that achieves sub-100-molecule sensitivity using engineered kinetic proofreading, outperforming existing methods for Alzheimer's and SARS-CoV-2 biomarkers.

bioRxiv · Oct 7, 2026 · PreprintBioengineering

Structural Brain Indicators of Cognitive Performance in Middle and Late Adulthood: The Human Connectome Project in Aging/Aging Adult Brain Connectome Cohort

Yue Hong, Christa B Michel, Gabriele Maria Gassner, Courtney Accorsi, Beau M. Ances, Lauren M Antonucci, Steven Edward Arnold, Ganesh Muneshwar Babulal, Susan Y. Bookheimer, Randy L. Buckner, Carlos Cruchaga, Mirella Díaz‐Santos, Jennifer Stine Elam, David Van Essen, Dara G. Ghahremani, , David H. Salat

This study identifies structural brain differences associated with cognitive performance in middle and late adulthood using data from the Human Connectome Project.

bioRxiv · Jan 22, 2026 · PreprintNeuroscience

Identification of Novel Biomarkers for Alzheimer's Disease and Related Dementias Using Unbiased Plasma Proteomics

Benjamin Lacar, Shadi Ferdosi, Amir Alavi, Alexey Stukalov, Guhan Ram Venkataraman, Matthijs de Geus, Hiroko Hayama Dodge, Chao‐Yi Wu, Pia Kivisäkk, Sudeshna Das, Harendra Guturu, Brad T. Hyman, Serafim Batzoglou, Steven Edward Arnold, Asim S. Siddiqui

This study identifies novel plasma biomarkers for Alzheimer's disease and related dementias using unbiased proteomics in a large cohort.

bioRxiv · Jan 5, 2024 · PreprintNeuroscience

Cryopreservation of cerebrospinal fluid cells preserves transcriptomics integrity for single-cell analysis

Mahesh Chandra Kodali, Jerry Antone, Eric B. Alsop, Rojashree Jayakumar, Khushi Parikh, Paula Sanchez-Molina, Bahareh Ajami, Steven Edward Arnold, Kendall Jensen, Sudeshna Das, Marc S. Weinberg

This study shows that cryopreservation of CSF cells using FBS/DMSO or Recovery media preserves transcriptomic integrity for single-cell analysis, enabling accurate cell type detection and gene expression profiling.

bioRxiv · Aug 10, 2023 · PreprintNeuroscience

Multi-ancestry genome-wide meta-analysis of 56,241 individuals identifies LRRC4C, LHX5-AS1 and nominates ancestry-specific loci PTPRK, GRB14, and KIAA0825 as novel risk loci for Alzheimer disease: the Alzheimer Disease Genetics Consortium

Farid Rajabli, Penelope H. Benchek, Giuseppe Tosto, Nicholas A. Kushch, Jin Sha, Katrina Bazemore, Congcong Zhu, Wan‐Ping Lee, Jacob Haut, Kara L. Hamilton‐Nelson, Nicholas R. Wheeler, Yi Zhao, John J. Farrell, Michelle A. Grunin, Yuk Yee Leung, , Matthew P. Frosch

This study identifies novel Alzheimer's disease risk loci by analyzing multi-ancestry GWAS data from diverse populations, highlighting the importance of including underrepresented groups in genetic research.

medRxiv · Jul 6, 2023 · PreprintGenetic & Genomic Medicine

Manifestations of genetic risk for Alzheimer's Disease in the blood: a cross-sectional multi-omic analysis in healthy adults aged 18-90+

Laura M. Heath, John C. Earls, Andrew T. Magis, Sergey A. Kornilov, Jennifer C. Lovejoy, Cory C. Funk, Noa Rappaport, Benjamin A. Logsdon, Lara M. Mangravite, Brian W. Kunkle, Eden R. Martin, Adam Christian Naj, Nilüfer Ertekin‐Taner, Todd Eliot Golde, Leroy Hood, , Jeffrey Alan Kaye

This study identifies blood-based biomarkers associated with Alzheimer's disease genetic risk variants in a large cohort of healthy adults, showing effects from early adulthood and sex-specific patterns.

Scientific Reports · Apr 12, 2022Peer-reviewedGenetics

Multiplexed fractionated proteomics reveals synaptic factors associated with cognitive resilience in Alzheimer's Disease

Becky Catherine Carlyle, Savannah E. Kandigian, Johannes Kreuzer, Sudeshna Das, Bianca A. Trombetta, Yikai Kuo, David Alan Bennett, Julie A. Schneider, Vladislav Petyuk, Robert R. Kitchen, Robert Tyler Morris, Angus Clark Nairn, Bradley T. Hyman, Wilhelm Haas, Steven Edward Arnold

The paper uses multiplexed proteomics to identify synaptic factors linked to cognitive resilience in Alzheimer's Disease by analyzing brain tissue from individuals with varying pathology and cognitive outcomes.

bioRxiv · Jul 31, 2020 · PreprintNeuroscience

Prion protein quantification in cerebrospinal fluid as a tool for prion disease drug development

Sonia M. Vallabh, Chloe K. Nobuhara, Franc Llorens, Inga Zerr, Piero Parchi, Sabina Capellari, Eric W. Kuhn, Jacob Aaron Klickstein, Jiri Safar, Flávia C. Nery, Kathryn J. Swoboda, Michael D. Geschwind, Henrik Zetterberg, Steven Edward Arnold, Eric Vallabh Minikel, Stuart L. Schreiber

This study evaluates ELISA-based quantification of PrP in CSF as a biomarker for PrP-reducing therapeutics, showing its reliability and CNS origin.

Proceedings of the National Academy of Sciences · Apr 1, 2019Peer-reviewedMolecular Biology