CRISPR/Cas9-mediated genome editing reveals the involvement of a polyphenol oxidase in the shikonin-specific biosynthesis in Lithospermum erythrorhizon
Kohei Nakanishi, Yuki Takano, Kyoko Yamamoto, Yuki Matsuda, Mariko Yano, Koji Mito, Takuji Ichino, Kanade Tatsumi, Hao Li, Kazuaki Ohara, Ryosuke Munakata, Hideyuki Suzuki, Nozomu Sakurai, Daisuke Shibata, Keishi Osakabe, , Kazufumi Yazaki
The paper uses CRISPR/Cas9 to edit the LePPO1 gene in Lithospermum erythrorhizon, showing it is involved in shikonin biosynthesis and explains the role of copper in the process.
Plant and Cell Physiology · May 28, 2026Peer-reviewedBiochemistry