Keishi Osakabe

The paper uses CRISPR/Cas9 to edit the LePPO1 gene in Lithospermum erythrorhizon, showing it is involved in shikonin biosynthesis and explains the role of copper in the process.

This study investigates the role of C4H in rice lignin biosynthesis, showing it is essential but dispensable due to the compensatory PTAL pathway.

The paper shows that disrupting CAld5H and PMT genes in rice eliminates syringyl lignin, revealing a grass-specific pathway for p-coumarate conjugates and lineage-dependent lignin polymerization.

This study identifies Cas10d as a multi-functional nuclease in CRISPR type I-D system, enabling targeted mutagenesis in human cells with specific deletion and insertion capabilities.