Culturomics from field-grown crop plants using dilution-to-extinction, two-step library preparation and amplicon sequencing
Eglantina Lopez‐Echartea, Nicholas S. Dusek, Mallory Misialek, Mohammad Al Mahmud‐Un‐Nabi, Riley Williamson, Komal Marathe, Barney A. Geddes
This paper optimizes a culturomics protocol to isolate and identify hundreds of bacterial isolates from plant microbiomes using dilution-to-extinction culturing and a two-step barcoding PCR strategy with NGS and DADA2.
Microbiology · Jun 17, 2025Peer-reviewedMicrobiology