Nicholas S. Dusek

The paper develops a high-throughput pipeline called VECTOR to evaluate and improve genetic engineering of undomesticated microbes using modular plasmid vectors and barcode-based screening.

This paper optimizes a culturomics protocol to isolate and identify hundreds of bacterial isolates from plant microbiomes using dilution-to-extinction culturing and a two-step barcoding PCR strategy with NGS and DADA2.

This study adapts Plasmid-ID technology to assess nitrogen-fixing effectiveness and competitiveness of Sinorhizobium strains in the Medicago system using Next-Generation Sequencing and GFP fluorescence.