Barney A. Geddes

The paper develops inducible nodulation gene reporters to identify rhizobial and non-rhizobial strains capable of expressing S. meliloti nod genes, aiding in synthetic nitrogen fixation in non-legumes.

This study investigates how host mutations affect rhizobial fitness by inoculating Medicago truncatula mutants with Sinorhizobium meliloti strains, revealing host-by-strain interactions and identifying key rhizobial genes involved in fitness responses.

This study evaluates fluorescent reporters driven by the nifH promoter in legume-rhizobia symbiosis, identifying sfYFP and sfCFP as reliable tools for quantifying nodulation traits and introducing a deep learning pipeline for automated analysis.

The paper develops a high-throughput pipeline called VECTOR to evaluate and improve genetic engineering of undomesticated microbes using modular plasmid vectors and barcode-based screening.

This paper demonstrates an automated two-step PCR library preparation method using an acoustic liquid handler, reducing costs and time while maintaining sequencing quality.

This paper optimizes a culturomics protocol to isolate and identify hundreds of bacterial isolates from plant microbiomes using dilution-to-extinction culturing and a two-step barcoding PCR strategy with NGS and DADA2.

This study adapts Plasmid-ID technology to assess nitrogen-fixing effectiveness and competitiveness of Sinorhizobium strains in the Medicago system using Next-Generation Sequencing and GFP fluorescence.

BEVA2.0 expands the modular vector assembly platform with new parts and cloning plasmids, enhancing its utility for genetic engineering in proteobacteria and compatibility with other libraries.