Environmental activity-based protein profiling for function-driven enzyme discovery from natural communities
S. Ninck, T. Klaus, T. V. Kochetkova, S. P. Esser, L. Sewald, F. Kaschani, C. Braesen, A. J. Probst, I. Kublanov, B. Siebers, M. Kaiser
This paper introduces eABPP, a proteomics-based method to identify active enzymes in microbial communities, and demonstrates its application in discovering thermostable serine hydrolases from hot springs.
bioRxiv · Nov 11, 2022 · PreprintBiochemistry