Protein-mediated genome folding allosterically enhances site-specific integration of foreign DNA into CRISPRs
A. Santiago-Frangos, W. S. Henriques, T. Wiegand, C. C. Gauvin, M. Buyukyoruk, A. B. Graham, R. A. Wilkinson, L. Triem, K. Neselu, E. T. Eng, G. C. Lander, B. Wiedenheft
The paper determines the structure of a CRISPR integration complex and explains how it facilitates site-specific DNA integration through DNA bending and loop formation.
bioRxiv · May 26, 2023 · PreprintBiochemistry