E. T. Eng

Early intermediates in bacterial RNA polymerase promoter melting visualized by time-resolved cryo-electron microscopy

This study uses time-resolved cryo-EM to visualize early intermediates in bacterial RNA polymerase promoter melting, revealing key steps in the formation of the transcription-competent open complex.

Protein-mediated genome folding allosterically enhances site-specific integration of foreign DNA into CRISPRs

The paper determines the structure of a CRISPR integration complex and explains how it facilitates site-specific DNA integration through DNA bending and loop formation.

Fully Automated Multi-Grid Cryo-EM Screening using Smart Leginon

Smart Leginon automates cryo-EM grid screening, reducing operator time from 6 hours to <10 minutes and improving throughput.

Structural basis for substrate selection by the SARS-CoV-2 replicase

This study uses cryo-EM to reveal how the SARS-CoV-2 RTC discriminates between natural NTPs and the antiviral remdesivir triphosphate, providing insights for antiviral design.

Ensemble cryo-electron microscopy reveals conformational states of the nsp13 helicase in the SARS-CoV-2 helicase replication-transcription complex

This study uses cryo-EM and simulations to identify four conformational states of the nsp13 helicase in the SARS-CoV-2 RTC, revealing an allosteric mechanism for switching between RNA synthesis and backtracking.

System Environmental Metrics Collector for EM facilities

The paper develops a system for monitoring environmental metrics in cryoEM facilities to optimize instrument uptime and data production.

Structural origins of Escherichia coli RNA polymerase open promoter complex stability

This study determines the structural basis of RNA polymerase open complex stability in E. coli by analyzing three promoters with different lifetimes using cryo-EM.

Structural basis for backtracking by the SARS-CoV-2 replication-transcription complex

This paper shows that SARS-CoV-2 RdRp undergoes backtracking, which may aid proofreading and antiviral resistance.

Waffle Method: A general and flexible approach for FIB-milling small and anisotropically oriented samples

The paper introduces the Waffle Method to improve cryo-FIB/SEM workflows for preparing complex biological samples for cryo-ET, demonstrating its effectiveness with various samples and hardware.

Structural basis for helicase-polymerase coupling in the SARS-CoV-2 replication-transcription complex

The paper presents cryo-EM structures of the SARS-CoV-2 holo-RdRp complex with nsp13 helicase, revealing interactions and a new therapeutic target.

Time-resolved cryoEM using Spotiton

The paper introduces a method for preparing cryoEM grids to study short-lived molecular states by rapidly dispensing sample drops and vitrifying them.

Characterization of the SARS-CoV-2 S Protein: Biophysical, Biochemical, Structural, and Antigenic Analysis

This study evaluates the production, characterization, and antigenicity of SARS-CoV-2 S protein constructs in two cell lines, showing high-quality yields and consistent behavior across assays.

The impact of data reduction and lossy image formats on electron microscope images

This study examines how data compression and lossy image formats affect cryoEM reconstruction quality, finding that compressed data can still yield sub-2Å reconstructions.

High Resolution Single Particle Cryo-Electron Microscopy using Beam-Image Shift

This study evaluates the impact of beam-image shift on cryo-EM single-particle reconstruction quality by testing different beam tilt angles and analyzing FSC values and water density peaks.

Epitope-based vaccine design yields fusion peptide-directed antibodies that neutralize diverse strains of HIV-1

This study shows that targeting the HIV-1 fusion peptide with specific immunogens induces cross-clade neutralizing antibodies in animals.

Routine Determination of Ice Thickness for Cryo-EM Grids

The paper presents two methods to measure ice thickness in cryo-EM grids during data collection to improve sample preparation and data quality.

Routine Single Particle CryoEM Sample and Grid Characterization by Tomography

This study uses fiducial-less tomography to analyze cryoEM grids and finds that 90% of particles are adsorbed to air-water interfaces, impacting protein structure and single particle analysis.