High-depth whole genome sequencing of blood culture plates reveals evolutionary dynamics in cases of persistent bacteremia due to methicillin-resistant Staphylococcus aureus
Emma G. Mills, Leah M. Grady, Edwin Chen, Marla G. Shaffer, Ryan K. Shields, Daria Van Tyne, Matthew J. Culyba
This study compares high-depth whole genome sequencing of pooled (p-seq) and individual colonies (c5-seq) from blood culture plates to understand evolutionary dynamics in MRSA bacteremia, finding that c5-seq is more practical for tracking within-host evolution.
bioRxiv · Apr 27, 2026 · PreprintMicrobiology