Cell BiologyMolecular Biology of the CellPeer-reviewed
Single-cell quantitation of histones and histone post-translational modifications across the cell cycle by high-throughput imaging
Linda Karine Zane, Fleur Chapus, Gianluca Pegoraro, Tom Misteli
This paper introduces HiHiMap, a high-throughput imaging technique to quantify histone and PTM levels across the cell cycle at the single-cell level, revealing differences in PTM behavior between normal, immortalized, and transformed cells.