Structural Basis for Cas9-Directed Spacer Acquisition in Type II-A CRISPR-Cas Systems
Zhaoxing Li, Yutao Li, Jianping Kong, Qianqian Wu, Ping Huang, Yu Zhang, Wanqian Wu, Meirong Chen, Y.H. Liu, Hanfeng Lin, Liqiu Hou, Gongyu Liu, Ting Zeng, Yutong He, Chunyi Hu, , Yibei Xiao
This study reveals the structural dynamics of Cas9-mediated spacer acquisition in Type II-A CRISPR-Cas systems through cryo-EM analysis of the Cas9-Csn2-Cas1-Cas2 complex.
Molecular Cell · Feb 16, 2026Peer-reviewedMolecular Biology