Direct and culture-independent detection of low-abundant Clostridioides difficile in environmental DNA via PCR
M. A. Schuler, D. Schneider, A. Poehlein, R. Daniel
This paper develops a PCR method to detect low-abundant Clostridioides difficile in environmental samples, revealing biases in strain isolation due to antibiotic-based enrichment.
bioRxiv · Jul 24, 2023 · PreprintMicrobiology