Catalytically inactive dKbCas12d guided by sgRNA and new insights into its binding through a single-molecule approach
A. Alekseev, V. Klimko, I. Frantsuzova, M. Abramova, V. Vinnik, A. Vasileva, P. Selkova, M. Khodorkovskii, A. Arseniev
The paper describes modifications to the CRISPR-Cas12d system, identifies catalytically inactive variants, and demonstrates their high specificity using single-molecule techniques.
bioRxiv · Dec 12, 2023 · PreprintBiophysics