A. Alekseev

CryoRhodopsins: a comprehensive characterization of a group of microbial rhodopsins from cold environments

The paper characterizes CryoRhodopsins from cold environments, revealing their dual functionality and structural adaptations to low temperatures.

Catalytically inactive dKbCas12d guided by sgRNA and new insights into its binding through a single-molecule approach

The paper describes modifications to the CRISPR-Cas12d system, identifies catalytically inactive variants, and demonstrates their high specificity using single-molecule techniques.

Efficient and sustained optogenetic control of sensory and cardiac systems

This paper introduces ChReef, an improved optogenetic tool with enhanced performance in sensory and cardiac systems, demonstrating its effectiveness in restoring visual and auditory function.

A novel subgroup of light-driven sodium pumps with an additional Schiff base counterion

The paper identifies a new subgroup of light-driven sodium pumps with an additional glutamic acid residue that reduces pH sensitivity for sodium transport.

Hijacking of internal calcium dynamics by intracellularly residing rhodopsins

The paper shows that viral channelrhodopsins (VCR1s) induce intracellular calcium release upon illumination, enabling light-controlled muscle contraction in tadpoles, suggesting a new optogenetic tool.

Revealing the Oligomerization of Channelrhodopsin-2 in the Cell Membrane using Photo-Activated Localization Microscopy

This study uses qPALM to show ChR2 predominantly forms dimers in the cell membrane, with disulfide bonds not essential for dimerization and partial monomerization observed at low concentrations.

Flotillin-Associated rhodopsin (FArhodopsin), a widespread paralog of proteorhodopsin in aquatic bacteria with streamlined genomes

The paper identifies a new class of rhodopsins, FArhodopsins, found in marine and freshwater microbes, associated with membrane microdomains and potentially retinal-less function.

Viral channelrhodopsins: calcium-dependent Na+/K+ selective light-gated channels

The paper characterizes two viral channelrhodopsins, showing they are Ca2+-dependent Na+/K+ channels with unique optogenetic properties and structural differences from known channelrhodopsins.

Molecular mechanism of light-driven sodium pumping

The paper elucidates the molecular mechanism of Na+ pumping by KR2 rhodopsin using crystal structures and molecular dynamics, revealing a switching mechanism and structural similarities to channelrhodopsins.

High Resolution Structural Insights into Heliorhodopsin Family

The paper presents high-resolution structures of two HeR-48C12 states, revealing unique features and suggesting potential enzymatic activity and involvement in redox processes.

Viral etiology of Acute Respiratory Infections in Hospitalized Children in Novosibirsk City, Russia (2013 - 2017)