HDX-MS performed on BtuB in E. coli outer membranes delineates the luminal domain's allostery and unfolding upon B12 and TonB binding
A. M. Zmyslowski, M. C. Baxa, I. A. Gagnon, T. R. Sosnick
This study uses HDX-MS on BtuB in E. coli outer membranes to show B12 binding destabilizes the Ionic Lock, while TonB binding has weaker effects and no pore formation is observed.
bioRxiv · Jan 7, 2022Biochemistry