Live-cell imaging of circadian clock protein dynamics in CRISPR-generated knock-in cells
C. H. Gabriel, M. del Olmo, A. Zehtabian, S. Reischl, H. van Dijk, B. Koller, A. Grudziecki, B. U. Maier, H. Ewers, H. Herzel, A. E. Granada, A. Kramer
The paper develops a CRISPR-based method to generate knock-in cells for circadian clock proteins, revealing unexpected nuclear dynamics and rhythmic patterns of CRY1 and PER2.
bioRxiv · Feb 28, 2020Cell Biology