H. Ewers

Septin-associated PIPKIγ splice variants drive centralspindlin association with the midbody via PI(4,5)P2

This study identifies septin-associated PIPKIγ splice variants as essential for midbody organization by generating PI(4,5)P2 required for centralspindlin recruitment and septin-microtubule association.

Circadian period is compensated for repressor protein turnover rates in single cells

The paper investigates how circadian period compensates for fluctuations in repressor protein turnover rates in single cells using fluorescently labeled proteins and mathematical modeling.

ARF1 compartments direct cargo flow via maturation into recycling endosomes

The paper identifies two functionally distinct ARF1 compartments involved in post-Golgi sorting and endocytic recycling, challenging the classical vesicle shuttling model.

An optogenetic method for the controlled release of single molecules

This paper develops an optogenetic method to release single molecules in live cells, enabling controlled delivery of functional proteins and reconstitution of cellular functions at the single molecule level.

Sub-membrane actin rings compartmentalize the plasma membrane

This paper shows that sub-membrane actin rings compartmentalize the plasma membrane by confining membrane protein motion in a periodic pattern, observed in neurons and other cell types.

Adhesion energy controls lipid binding-mediated endocytosis

The paper shows that multivalent lipid binding by globular particles induces membrane deformation and clathrin-independent endocytosis, with adhesion energy determining the threshold for internalization.

An open-source, high resolution, automated fluorescence microscope

The paper presents an open-source, high-resolution fluorescence microscope that enables high-quality imaging at a low cost.

Endosome dysfunction leads to gain-of-function TLR7 and human lupus

This study shows that endosome dysfunction causes TLR7 gain-of-function and contributes to human lupus by disrupting TLR7 turnover and leading to self-reactivity.

Precise measurement of nanoscopic septin ring structures in deep learning-assisted quantitative superresolution microscopy

The paper uses deep learning and superresolution microscopy to analyze septin ring structures, finding a trade-off between measurement accuracy and dynamic range and showing septin ring size correlates with subunit count.

Glypican-1 drives unconventional secretion of Fibroblast Growth Factor 2

This study identifies Glypican-1 as the key factor driving unconventional secretion of FGF2, revealing its structural requirements and implications for tumor progression.

Cholesterol promotes both head group visibility and clustering of PI(4,5)P2 driving unconventional secretion of Fibroblast Growth Factor 2

This study shows cholesterol enhances FGF2 secretion by promoting PI(4,5)P2 clustering and membrane pore formation.

Directed manipulation of membrane proteins by fluorescent magnetic nanoparticles

This study uses fluorescent magnetic nanoparticles to manipulate and track membrane proteins in live cells, revealing submembrane barriers linked to the actin cytoskeleton.

Left-handed DNA-PAINT for improved superresolution imaging in the nucleus.

The paper introduces left-handed DNA-PAINT to improve superresolution imaging in the nucleus by avoiding interference from natural DNA.

Live-cell imaging of circadian clock protein dynamics in CRISPR-generated knock-in cells

The paper develops a CRISPR-based method to generate knock-in cells for circadian clock proteins, revealing unexpected nuclear dynamics and rhythmic patterns of CRY1 and PER2.

The α1 subunit of the Na,K-ATPase acts upstream of PI(4,5)P2 facilitating unconventional secretion of Fibroblast Growth Factor 2 from tumor cells

This study identifies a direct interaction between FGF2 and the Na,K-ATPase α1 subunit, showing it facilitates FGF2 membrane translocation upstream of PI(4,5)P2.

A homozygous genome-edited Sept2-EGFP fibroblast cell line

The paper generates a homozygous genome-edited rat fibroblast cell line expressing Sept2-EGFP from both chromosomal loci and validates its proper function and localization.

Rapid and efficient C-terminal labeling of nanobodies for DNA-PAINT

The paper develops an optimized method for labeling nanobodies with DNA oligomers for DNA-PAINT, enabling efficient and straightforward superresolution imaging.

Expansion stimulated emission depletion microscopy (ExSTED)

The paper combines expansion microscopy with STED to achieve 30-fold higher resolution than conventional microscopy, enabling ultra-structural imaging at <10 nm lateral and ~50 nm isotropic.