Bicistronic expression and differential localization of proteins in insect cells and Drosophila suzukii using picornaviral 2A peptides
J. Schwirz, Y. Yan, Z. Franta, M. F. Schetelig
This paper tests four picornaviral 2A peptides for co-expression and differential localization of proteins in insect cells and Drosophila suzukii, finding that EGFP-CAAX can be bred to homozygosity while DsRed-CAAX is lethal.
bioRxiv · Sep 12, 2019Bioengineering