A rationally designed and highly versatile epitope tag for nanobody-based purification, detection and manipulation of proteins
H. Goetzke, M. Kilisch, M. Martinez-Carranza, S. Sograte-Idrissi, A. Rajavel, T. Schlichthaerle, N. Engels, R. Jungmann, P. Stenmark, F. Opazo, S. Frey
The paper introduces the ALFA-tag, a versatile epitope tag that outperforms existing tags and enables multiple applications in protein detection, purification, and manipulation.
bioRxiv · Jun 7, 2019Biochemistry