R. Jungmann

Compliant DNA Origami Nanoactuators as Size-Selective Nanopores

This paper introduces a DNA origami-based nanopore that can adjust its size through molecular triggers, enabling size-selective transport of biomolecules.

The human-restricted oncoprotein POU5F1B enhances cell invasiveness through plasma membrane remodeling

This study identifies POU5F1B as a human-specific oncoprotein that enhances cell invasiveness via plasma membrane remodeling through ubiquitination and S-acylation.

Structure and regulation of GSDMD pores at the plasma membrane of pyroptotic cells

The paper visualizes GSDMD nanostructures at the plasma membrane of pyroptotic cells and identifies PI(3,4,5)P3 as a regulator of pore formation.

Simultaneous multicolor fluorescence imaging using duplication-based PSF splitting

The paper introduces a method to enhance fluorescence imaging by splitting the PSF into multiple copies, enabling simultaneous multicolor imaging with super-resolution and single-molecule capabilities.

DNA-PAINT single-particle tracking (DNA-PAINT-SPT) enables extended single-molecule studies of membrane protein interactions

This paper introduces dual-colour DNA-PAINT-SPT for live cell imaging, enabling tracking of membrane protein interactions and dimerisation with improved performance over single-dye SPT.

Simultaneous multicolor DNA-PAINT without sequential fluid exchange using spectral demixing

This paper introduces a method for simultaneous multicolor DNA-PAINT imaging without fluid exchange, achieving high localization precision and multicolor registration.

Nanoscale organization of the endogenous ASC speck

This study uses super-resolution microscopy to analyze the nanoscale structure of endogenous ASC specks during NLRP3 inflammasome activation, revealing a dense core with extending filaments and changes in density during formation.

Calibration free counting of low molecular copy numbers in single DNA-PAINT localization clusters

This paper introduces lbFCS+, a method to absolutely count molecular copy numbers in DNA-PAINT localization clusters without calibration.

Nanoscale 3D DNA tracing in single human cells visualizes loop extrusion directly in situ

This study uses nanoscale 3D imaging to show that DNA folds as flexible coils without loop extruding enzymes, but forms reproducible loops with Cohesin and CTCF.

Principles of RNA recruitment to viral ribonucleoprotein condensates in a segmented dsRNA virus

The paper investigates how rotavirus transcripts are recruited into viroplasms, showing that RNA enrichment depends on conserved terminal regions and varies among transcripts.

DNA origami demonstrate the unique stimulatory power of single pMHCs as T-cell antigens

The paper demonstrates that single pMHCs can robustly stimulate T-cells as isolated entities, identifying the minimal requirements for effective TCR-triggering.

Rotavirus Replication Factories Are Complex Ribonucleoprotein Condensates

This study shows rotavirus replication factories are RNA-protein condensates formed by phase separation, with unique features that could be targeted for antiviral therapies.

Direct Supercritical Angle Localization Microscopy for Nanometer 3D Superresolution

This paper introduces SALM, a 3D superresolution technique that improves z-resolution by four times using direct splitting of supercritical and undercritical emission.

Tracking Single Particles for Hours via Continuous DNA-mediated Fluorophore Exchange

This paper introduces a method to track single particles for hours using continuous DNA-mediated fluorophore exchange, overcoming photobleaching limitations.

Nanoscale pattern extraction from relative positions of sparse 3D localisations

The paper introduces a method to extract high-resolution ordered features from localisation microscopy data by analyzing relative molecular positions, applied to infer ultrastructure of various biological structures.

Circumvention of common labeling artifacts using secondary nanobodies

This paper demonstrates that monovalent nanobodies improve imaging accuracy and reduce artifacts compared to traditional secondary antibodies in super-resolution microscopy.

Sub-Nanometer Precision using Bayesian Grouping of Localizations

The paper introduces a Bayesian method to improve localization precision in single-molecule microscopy by grouping localizations from multiple blinking events.

A rationally designed and highly versatile epitope tag for nanobody-based purification, detection and manipulation of proteins

The paper introduces the ALFA-tag, a versatile epitope tag that outperforms existing tags and enables multiple applications in protein detection, purification, and manipulation.

Direct visualization of single nuclear pore complex proteins using genetically-encoded probes for DNA-PAINT

This paper develops a method to directly visualize single nuclear pore complex proteins using DNA-PAINT microscopy with genetically-encoded tags, achieving ~3 nm resolution in human cells.

Bayesian Multiple Emitter Fitting using Reversible Jump Markov Chain Monte Carlo

The paper introduces a Bayesian method using Reversible Jump Markov Chain Monte Carlo to fit multiple emitters in dense super-resolution imaging data, providing posterior distributions and model coordinates.

Rapid Sequential In Situ Multiplexing With DNA-Exchange-Imaging

This paper introduces DNA-Exchange-Imaging, a method for rapid, highly multiplexed in situ protein visualization, achieving sub-20 nm resolution and reducing imaging time from days to minutes.