T. Schlichthaerle

Modulation of FGF pathway signaling and vascular differentiation using designed oligomeric assemblies

This study designs synthetic oligomeric ligands to modulate FGFR signaling and shows their impact on vascular cell fate decisions.

Design of High Affinity Binders to Convex Protein Target Sites

This paper designs high-affinity proteins that bind to convex protein sites using geometrically matching concave scaffolds, achieving low nanomolar to picomolar affinities.

Simultaneous multicolor fluorescence imaging using duplication-based PSF splitting

The paper introduces a method to enhance fluorescence imaging by splitting the PSF into multiple copies, enabling simultaneous multicolor imaging with super-resolution and single-molecule capabilities.

DNA origami demonstrate the unique stimulatory power of single pMHCs as T-cell antigens

The paper demonstrates that single pMHCs can robustly stimulate T-cells as isolated entities, identifying the minimal requirements for effective TCR-triggering.

Nanoscale pattern extraction from relative positions of sparse 3D localisations

The paper introduces a method to extract high-resolution ordered features from localisation microscopy data by analyzing relative molecular positions, applied to infer ultrastructure of various biological structures.

Circumvention of common labeling artifacts using secondary nanobodies

This paper demonstrates that monovalent nanobodies improve imaging accuracy and reduce artifacts compared to traditional secondary antibodies in super-resolution microscopy.

A rationally designed and highly versatile epitope tag for nanobody-based purification, detection and manipulation of proteins

The paper introduces the ALFA-tag, a versatile epitope tag that outperforms existing tags and enables multiple applications in protein detection, purification, and manipulation.

Direct visualization of single nuclear pore complex proteins using genetically-encoded probes for DNA-PAINT

This paper develops a method to directly visualize single nuclear pore complex proteins using DNA-PAINT microscopy with genetically-encoded tags, achieving ~3 nm resolution in human cells.

Bayesian Multiple Emitter Fitting using Reversible Jump Markov Chain Monte Carlo

The paper introduces a Bayesian method using Reversible Jump Markov Chain Monte Carlo to fit multiple emitters in dense super-resolution imaging data, providing posterior distributions and model coordinates.