Immortalization and transformation of primary cells mediated by engineered ecDNAs
D. Pradella, M. Zhang, R. Gao, Y. A. Melissa, K. M. Gluchowska, Y. Cendon Florez, T. Mishra, G. La Rocca, M. Weigl, Z. Jiao, H. H. M. Nguyen, F. Grimm, M. Lisi, C. Mastroleo, K. Chen, , A. Ventura
This paper describes a method to engineer large focal amplifications in primary cells using ecDNAs, showing they promote proliferation and transformation.
bioRxiv · Jun 25, 2023 · PreprintCancer Biology