Human pluripotent reprogramming with CRISPR activators
J. Weltner, D. Balboa, S. Katayama, M. M. Bespalov, K. Krjutskov, E.-M. Jouhilahti, R. Trokovic, J. Kere, T. Otonkoski
This study reprograms human skin fibroblasts into iPSC using CRISPRa, showing that targeting an Alu-motif enhances reprogramming efficiency by improving activation of NANOG and REX1.
bioRxiv · Oct 19, 2017Cell Biology